Laboratory of Enzyme Chemistry
| Adress: 690022 Vladivostok, prospekt 100-let Vladivostoku, 159
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| tel.+7 (423)231-07-05
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| Fax. +7(423)231-07-05 (additional 7) |
The head of the Laboratory:
Svetlana Ermakova
Doctor of Chemicals Sciences
swetlana_e@mail.ru
Research staff:
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Mikhail Kusaykin
Ph.D., DrSc. Associate professor
Deputy Director
mik@piboc.dvo.ru
Tel. +7423231-07-05, additional 1
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Irina Bakunina
Ph.D., DrSc, Senior researcher
Tel.+7423231-07-05, additional 3
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Artem Silchenko
Ph.D., Senior researcher
(tel. 231-07-05, additional 5) |
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| Yuliya Burtseva
Ph.D., Senior researcher burtseva@piboc.dvo.ru (tel. 231-07-05, additional 5) |
Svetlana Ermakova
Ph.D., Senior researcher swetlana_e@mail.ru, svetlana@piboc.dvo.ru (tel. 231-07-05, additional 4) |
Natalia Shevchenko
Ph.D., Senior researcher (tel. 231-07-05, additional 6) |
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Olesya Vischuk
Ph.D., Junior researcher (tel. 231-07-05, additional 4) | Marina Kiseleva
Researcher |
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| Ludmila Elyakova
Dsc., professor (tel. Moscow (495)336-82-33 |
Rosa Menshova
Junior researcher Ph.D. | |
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Maria Pesentseva
Junior researcher dolphy-marisha@list.ru (tel. 231-07-05, additional 1) |
Imbs Tatyana Igorevna
Ph.D. |
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Artem Silchenko
Ph.D. student | Students |
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A living system controls its activity through enzymes. An enzyme is a protein molecule that is a biological catalyst with three characteristics. First, the basic function of an enzyme is to increase the rate of a reaction. Most cellular reactions occur about a million times faster than they would in the absence of an enzyme. Second, most enzymes act specifically with only one reactant (called a substrate) to produce products. The third and most remarkable characteristic is that enzymes are regulated from a state of low activity to high activity and vice versa. Gradually, you will appreciate that the individuality of a living cell is due in large part to the unique set of some 3,000 enzymes that it is genetically programmed to produce. If even one enzyme is missing or defective, the results can be disastrous.
We isolate the enzymes from marine macro- and microorganisms. Our Lab mainly focus on enzymes cleaving polysaccharides from brown seaweeds: 1,3-β-D-glucanases, fucoidanases, sulfatases, a-galactosidases, alginate lyases
The main goals:
Structure and function of biological macromolecules (enzymes and polysaccharides).
The mechanism of action of enzymes and biotechnological aspects of their application.
The work is performed by coordination between three groups of our Lab:
“E-group” – enzyme investigation (isolation, structure and function elucidation, cloning of candidate genes, recombinant protein production);
“S-group” – substrate investigation (isolation of the polysaccharides, structure elucidation);
“B-group” – bioactivity investigation (cytotoxicity and anti-cancer activity of the polysaccharides and the products of enzymatic reaction).
Highlights and perspectives:
Characterization of the recombinant fucoidanase
Crystallization and 3D structure of the glucanase from Pseudocardium sacchalinensis
Lab staff:
Victoria Sova (senior researcher, Ph.D/E-group), Yulya Dubrovskaya (researcher, Ph.D/E-group), Irina Bakunina (senior researcher, D.Sci./E-group), Olesya Vishchuk (junior researcher, Ph.D./B-group), Svetlana Ermakova (senior researcher, Ph.D/B-group), Natalya Shevchenko (senior researcher, Ph.D/S-group), Rose Men’shova (junior researcher, Ph.D/S-group), Tatyana Imbs (researcher, Ph.D./S-group), Marina Kiseleva (researcher/B-group), Maria Pesentseva (junior researcher/E-group), Mikhail Kusaykin (deputy Director, Ph.D./E-group), Artem Silchenko (Ph.D. student/E-group)
Scientific collaborations
Our laboratory conduct joint research with other institutes such as Institute of chemistry, Insitute of biology and soil, Pasific scientific research fisheries center, Institute of epidemiology and microbiology (Vladivostok), Institute of biophysics (Moscow), N.D. Zelinsky Institute of organic chemistry RAS, Institute of cancer (Kiev, Ukraine), National research center for haematology (Moscow), ND SRI Clinikal and experemental limphology SB RAMS(Novosibirsk).