Laboratory of Enzyme Chemistry

Adress: 690022 Vladivostok,prospekt 100-let Vladivostoku, 159
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tel.+7 (423)231-07-05


Fax. +7(423)231-07-05 (additional 7)

The head of the Laboratory:

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Svetlana Ermakova

Doctor of Chemicals Sciences
swetlana_e@mail.ru

Research staff:

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Mikhail Kusaykin
Ph.D., DrSc. Associate professor
Deputy Director
mik@piboc.dvo.ru
Tel. +7423231-07-05, additional 1
Irina Bakunina
Ph.D., DrSc, Senior researcher
Tel.+7423231-07-05, additional 3
Artem Silchenko
Ph.D., Senior researcher
(tel. 231-07-05, additional 5)
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YuliyaBurtseva
Ph.D., Senior researcher
burtseva@piboc.dvo.ru
(tel. 231-07-05, additional 5)


SvetlanaErmakova
Ph.D., Senior researcher
swetlana_e@mail.ru,
svetlana@piboc.dvo.ru
(tel. 231-07-05, additional 4)
NataliaShevchenko
Ph.D., Senior researcher
(tel. 231-07-05, additional 6)


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OlesyaVischuk
Ph.D., Junior researcher
(tel. 231-07-05, additional 4)
Marina Kiseleva
Researcher


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LudmilaElyakova
Dsc., professor
(tel. Moscow (495)336-82-33
RosaMenshova
Junior researcher
Ph.D.
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MariaPesentseva
Junior researcher
dolphy-marisha@list.ru
(tel. 231-07-05, additional 1)
Imbs Tatyana Igorevna

Ph.D.





Artem Silchenko

Ph.D. student
Students











































































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A living system controls its activity through enzymes. An enzyme is a protein molecule that is a biological catalyst with three characteristics. First, the basic function of an enzyme is to increase the rate of a reaction. Most cellular reactions occur about a million times faster than they would in the absence of an enzyme. Second, most enzymes act specifically with only one reactant (called a substrate) to produce products. The third and most remarkable characteristic is that enzymes are regulated from a state of low activity to high activity and vice versa. Gradually, you will appreciate that the individuality of a living cell is due in large part to the unique set of some 3,000 enzymes that it is genetically programmed to produce. If even one enzyme is missing or defective, the results can be disastrous.

We isolate the enzymes from marine macro- and microorganisms. Our Lab mainly focus on enzymes cleaving polysaccharides from brown seaweeds: 1,3-β-D-glucanases, fucoidanases, sulfatases, a-galactosidases, alginate lyases

The main goals:

Structure and function of biological macromolecules (enzymes and polysaccharides).

The mechanism of action of enzymes and biotechnological aspects of their application.

The work is performed by coordination between three groups of our Lab:

“E-group” – enzyme investigation (isolation, structure and function elucidation, cloning of candidate genes, recombinant protein production);

S-group substrate investigation (isolation of the polysaccharides, structure elucidation);

B-group bioactivity investigation (cytotoxicity and anti-cancer activity of the polysaccharides and the products of enzymatic reaction).

Highlights and perspectives:

Characterization of the recombinant fucoidanase

Crystallization and 3D structure of the glucanase from Pseudocardium sacchalinensis

Lab staff:

Victoria Sova (senior researcher, Ph.D/E-group), Yulya Dubrovskaya (researcher, Ph.D/E-group), Irina Bakunina (senior researcher, D.Sci./E-group), Olesya Vishchuk (junior researcher, Ph.D./B-group), Svetlana Ermakova (senior researcher, Ph.D/B-group), Natalya Shevchenko (senior researcher, Ph.D/S-group), Rose Men’shova (junior researcher, Ph.D/S-group), Tatyana Imbs (researcher, Ph.D./S-group), Marina Kiseleva (researcher/B-group), Maria Pesentseva (junior researcher/E-group), Mikhail Kusaykin (deputy Director, Ph.D./E-group), Artem Silchenko (Ph.D. student/E-group)

Scientific collaborations

Our laboratory conduct joint research with other institutes such as Institute of chemistry, Insitute of biology and soil, Pasific scientific research fisheries center, Institute of epidemiology and microbiology (Vladivostok), Institute of biophysics (Moscow), N.D. Zelinsky Institute of organic chemistry RAS, Institute of cancer (Kiev, Ukraine), National research center for haematology (Moscow),ND SRI Clinikal and experemental limphologySB RAMS(Novosibirsk).

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